the regulatory role of the Hfq protein

the regulatory role of the Hfq protein

Interaction between Hfq protein and single-stranded DNA reveals the role of amyloid protein in gene recombination and replication

The regulatory role of the Hfq protein, a bacterial protein found mainly in Escherichia coli (Escherichia coli, also called colibacillus or E. coli, is a bacterium…), is mainly attributed to its function as an RNA chaperone, facilitating interactions between RNAs. the protein (A protein is a biological macromolecule composed of one or more…) it also participates in the structuring and compaction of DNA.

Extensive international collaboration has been studied using various techniques ofimages (Images are primarily the creation and trade of physical images that…) and spectroscopy, in particular on synchrotron (The term synchrotron refers to a large electromagnetic instrument intended…), the complexes formed by the amyloid protein Hfq and single-stranded DNA. This demonstrates that the Hfq protein may play a role in the genetic recombination (Genetic recombination is “the phenomenon that leads to…) and replication.

The Hfq protein is a bacterial regulator that is involved in many aspects of the metabolism (Metabolism is the set of molecular and energetic transformations…) nucleic acids (RNA and DNA), giving it many important physiological functions. Among these multiple functions, it is essential for the replication of the bacteriophage (A bacteriophage (or phage) is a virus that infects only bacteria. In Greek, phageton…) Qβ, RNA viruses (An RNA virus is a virus that uses RNA as its genetic material, or…) single-stranded positive, which infects bacteria such as Escherichia coli, bacterium (Bacteria (Bacteria) are prokaryotic unicellular living organisms, characterized…) municipality of the gut flora (The intestinal flora is the set of microorganisms found in the digestive tract. The…). The Hfq protein regulates (The rules are alloys of tin or lead and antimony). also the efficiency of mRNA translation and helps compact double-stranded DNA within bacterial cells. But these functions need to be better explored and specified, in particular the modes ofInteraction (An interaction is an exchange of information, affection or energy between two agents within…) that can bind to the Hfq protein and to DNA (single-stranded or double-stranded).

Two previous studies have shown that the Hfq protein:
– forms amyloid structures, such as those found in neurodegenerative diseases, and that these structures affect the integrity of the bacterial membrane.
– is involved in control (The word control can have several meanings. It can be used as a synonym of examination, of…) of the flow of a DNA solution. It is in particular the amyloid region (C-terminal) of the Hfq protein that is involved in the response mechanic (In everyday parlance, mechanics is the field of machines, engines, vehicles, organs, etc.) of liquid (A fluid is a perfectly deformable material medium. We group it under this…) complex, forming (In intonation, fundamental frequency changes are perceived as variations of…) an inhomogeneous “soft” solid.

These initial results were followed by a new structural study, showing for the first time that the amyloid region of Hfq radically alters the structure of single-stranded DNA by forming a propeller (Helix comes from a Greek word helix meaning “spiral”. A shaped object…) parallel, causing the Hfq:DNA complex to align.

To get this result a combination (A combination can be:) experimental methodologies that include spectroscopy (circular and linear synchrotron dichroism, infrared) and molecular imaging (fluorescence and microscopy (Microscopy is the observation of a sample (placed in a microscope slide…) electron) was used to probe the interactions between Hfq and single-stranded DNA and the nucleoprotein complexes formed.


Different views of the molecular representation of the Hfq protein: The molecular surface of the N-terminal domain is shown in gray and green (Green is a complementary color corresponding to light having a wavelength…). Beta sheets (blue) and alpha helix (red) are shown as a ribbon in a monomer. Here the C-terminal amyloid region is missing due to an unknown structure.

The action of the Hfq protein on DNA highlighted has important implications for DNA replication and recombination. Live.

Thus, Hfq single-stranded DNA-binding proteins play a crucial role in many fundamental biological processes, including DNA replication and recombination. genetic (Genetics (from the Greek genno γεννώ = to give birth) is…). Hfq is also a actor (An actor is an artist who embodies a character in a movie, in a play, in…) important to consider in the structure of bacterial chromosomes and the control of gene expression The demonstration of the participation of these amyloid proteins in genetic recombination reveals a new mode of regulation (The term regulation refers in its concrete sense to a technical discipline, which is…) possible replication and recombination, which can occur in all living cells.

Reference:
Amyloid-like Hfq interaction with single-stranded DNA: involvement in recombination and replication in Escherichia coli,
Krzysztof Kubiak, Frank Wien, Indresh Yadav, Nykola C. Jones, Søren Vrønning Hoffmann, Eric Le Cam, Antoine Cossa, Frederic Geinguenaud, Johan RC van der Maarel, Grzegorz Węgrzyn, and Véronique Arluison, will appear in Quarterly-Reviews-of-Biophysics.

Collaboration:
University (A university is an institution of higher education whose objective is the…) Paris-Saclay, Laboratory Leon Brillouin (Léon Brillouin (born August 7, 1889 in Sèvres – died in 1969 in New York) was a physicist…) UMR CEA-CNRS, LLB/MMB
– DISCO Beamline, Synchrotron SUN (The Sun (Sol in Latin, Helios or Ήλιος in Greek) is the star…)
– ISA Synchrotron (Aarhus Denmark) and Department of Physics and Astronomy, Aarhus University
– CNanoMat and Inserm platform, U1148, Vascular Translation Laboratory Sciences (Science (from the Latin scientia, “knowledge”) is, according to the dictionary…)UFR SMBH, University Paris 13 (Paris 13 University (official name: Paris-XIII) is a university…), City of Paris from the Sorbonne (Sorbonne Paris Cité is a Center for Research and Higher Education (PRES)…)University of Bobigny
– Department of Molecular Biology, University of Gdansk (Poland),
– Department of Physics, National University of Singapore
– Integrity of the genome and Cancer (Cancer is a disease characterized by abnormal cell proliferation…), Institute (An institute is a permanent organization created for a specific purpose. It is…) gustave roussy (Gustave Roussy, born on November 24, 1874 in Vevey (Switzerland) and died on…)
Curie Institute (The Institut Curie is a foundation, whose main activities are on the one hand…)PSL University, Paris-Saclay University, CNRS (The National Center for Scientific Research, better known by its acronym CNRS, is the largest…) UMS2016, Inserm US43, Multimodal Imaging Center.

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